A small amount of serum or solute can be serially diluted by transferring aliquots to diluent. coli in nutrient broth all the way to soil samples and hamburger meat. SERIAL DILUTIONS TUBE METHOD Principle Serial dilution is a common technique used in many immunologic procedures. Medical and pharmaceutical personnel are constantly dealing with dosages that require concentration measurements and dilutions. Serial Dilution Protocols Created:Friday, 30 September 2005 Author Jackie Reynolds Information It is a common practice to determine microbial counts for both liquid and solid specimens-suspensions of E. Of course, the addition of the stock solution affects the total volume of the diluted solution, but the final concentration is likely close enough even for medical purposes. If the stock solution is 10.0% KCl and the final volume and concentration need to be 100 mL and 0.50%, respectively, then it is easy to calculate how much stock solution to use: Broth dilution susceptibility test (MIC 6. At the end of the incubation period (generally 18-24 hours), the tubes are visually examined for turbidity. Medical personnel commonly must perform dilutions for IV solutions. Serial dilutions of the test agent are made in a liquid microbial growth medium inoculated with a standardized number of organisms and incubated for a prescribed time.
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